GEBR-7b, a novel PDE4D selective inhibitor that improves memory in rodents at non-emetic doses

Olga Bruno, Ernesto Fedele, Jos Prickaerts, L Parker, E Canepa, C Brullo, A Cavallero, E Gardella, A Balbi, C Domenicotti, Eva Bollen, Jérôme Gijselaers, Tim Vanmierlo, K Erb, C Limebeer, F Argellati, U Marinari, M Pronzato, R Ricciarelli

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    Abstract

    BACKGROUND AND PURPOSE Strategies designed to enhance cerebral cAMP have been proposed as symptomatic treatments to counteract cognitive deficits. However, pharmacological therapies aimed at reducing PDE4, the main class of cAMP catabolizing enzymes in the brain, produce severe emetic side effects. We have recently synthesized a 3-cyclopentyloxy-4-methoxybenzaldehyde derivative, structurally related to rolipram, and endowed with selective PDE4D inhibitory activity. The aim of the present study was to investigate the effect of the new drug, namely GEBR-7b, on memory performance, nausea, hippocampal cAMP and amyloid-b (Ab) levels. EXPERIMENTAL APPROACH To measure memory performance, we performed object recognition tests on rats and mice treated with GEBR-7b or rolipram. The emetic potential of the drug, again compared with rolipram, was evaluated in rats using the taste reactivity test and in mice using the xylazine/ketamine anaesthesia test. Extracellular hippocampal cAMP was evaluated by intracerebral microdialysis in freely moving rats. Levels of soluble Ab peptides were measured in hippocampal tissues and cultured N2a cells by ELISA. KEY RESULTS GEBR-7b increased hippocampal cAMP, did not influence Ab levels and improved spatial, as well as object memory performance in the object recognition tests. The effect of GEBR-7b on memory was 3 to 10 times more potent than that of rolipram, and its effective doses had no effect on surrogate measures of emesis in rodents. CONCLUSION AND IMPLICATIONS Our results demonstrate that GEBR-7b enhances memory functions at doses that do not cause emesis-like behaviour in rodents, thus offering a promising pharmacological perspective for the treatment of memory impairment.
    Original languageEnglish
    Pages (from-to)2054-2063
    Number of pages10
    JournalBritish Journal of Pharmacology
    Volume164
    Issue number8
    DOIs
    Publication statusPublished - Dec 2011

    Keywords

    • PDE
    • memory
    • rodents
    • emesis
    • LTP
    • cAMP

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