In vivo Toxicity Assessment of Silver Nanoparticles in Homeostatic versus Regenerating Planarians

Nathalie Leynen, Frank G.A.J. Van Belleghem, Annelies Wouters, Hannelore Bove, Jan-Pieter Ploem, Elsy Thijssen, Sabine A.S. Langie, Robert Carleer, Marcel Ameloot, Tom Artois, Karen Smeets*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Silver nanoparticles (AgNPs) belong to the most commercialized nanomaterials, used in both consumer products and medical applications. Despite its omnipresence, in-depth knowledge on the potential toxicity of nanosilver is still lacking, especially for developing organisms. Research on vertebrates is limited due to ethical concerns, and planarians are an ideal invertebrate model to study the effects of AgNPs on stem cells and developing tissues in vivo, as regeneration mimics development by triggering massive stem cell proliferation. Our results revealed a strong interference of AgNPs with tissue- and neuroregeneration which was related to an altered stem cell cycle. The presence of a PVP-coating significantly influenced toxicity outcomes, leading to elevated DNA-damage and decreased stem cell proliferation. Non-coated AgNPs had an inhibiting effect on stem cell and early progeny numbers. Overall, regenerating tissues were more sensitive to AgNP toxicity, and careful handling and appropriate decision making is needed in AgNP applications for healing and developing tissues. We emphasize on the importance of AgNP characterization, as we showed that changes in physicochemical properties influence toxicity.
Original languageEnglish
Pages (from-to)476-491
Number of pages16
JournalNanotoxicology
Volume13
Issue number4
Early online date14 Feb 2019
DOIs
Publication statusPublished - 21 Apr 2019

Fingerprint

Planarians
Stem cells
Silver
Nanoparticles
Toxicity
Stem Cells
Tissue
Cell proliferation
Cell Proliferation
Consumer products
Nanostructures
Medical applications
Invertebrates
Nanostructured materials
DNA Damage
Vertebrates
Regeneration
Decision Making
Cell Cycle
DNA

Keywords

  • silver nanoparticles
  • AgNPs
  • developmental toxicity
  • stem cell
  • coating
  • VITRO
  • BIOCOMPATIBILITY
  • STEM-CELL BIOLOGY
  • DNA-DAMAGE
  • ZINC-OXIDE
  • MODEL
  • PVP
  • GENOTOXICITY
  • DIFFERENTIATION
  • CYTOTOXICITY

Cite this

Leynen, Nathalie ; Van Belleghem, Frank G.A.J. ; Wouters, Annelies ; Bove, Hannelore ; Ploem, Jan-Pieter ; Thijssen, Elsy ; Langie, Sabine A.S. ; Carleer, Robert ; Ameloot, Marcel ; Artois, Tom ; Smeets, Karen. / In vivo Toxicity Assessment of Silver Nanoparticles in Homeostatic versus Regenerating Planarians. In: Nanotoxicology. 2019 ; Vol. 13, No. 4. pp. 476-491.
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abstract = "Silver nanoparticles (AgNPs) belong to the most commercialized nanomaterials, used in both consumer products and medical applications. Despite its omnipresence, in-depth knowledge on the potential toxicity of nanosilver is still lacking, especially for developing organisms. Research on vertebrates is limited due to ethical concerns, and planarians are an ideal invertebrate model to study the effects of AgNPs on stem cells and developing tissues in vivo, as regeneration mimics development by triggering massive stem cell proliferation. Our results revealed a strong interference of AgNPs with tissue- and neuroregeneration which was related to an altered stem cell cycle. The presence of a PVP-coating significantly influenced toxicity outcomes, leading to elevated DNA-damage and decreased stem cell proliferation. Non-coated AgNPs had an inhibiting effect on stem cell and early progeny numbers. Overall, regenerating tissues were more sensitive to AgNP toxicity, and careful handling and appropriate decision making is needed in AgNP applications for healing and developing tissues. We emphasize on the importance of AgNP characterization, as we showed that changes in physicochemical properties influence toxicity.",
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author = "Nathalie Leynen and {Van Belleghem}, {Frank G.A.J.} and Annelies Wouters and Hannelore Bove and Jan-Pieter Ploem and Elsy Thijssen and Langie, {Sabine A.S.} and Robert Carleer and Marcel Ameloot and Tom Artois and Karen Smeets",
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Leynen, N, Van Belleghem, FGAJ, Wouters, A, Bove, H, Ploem, J-P, Thijssen, E, Langie, SAS, Carleer, R, Ameloot, M, Artois, T & Smeets, K 2019, 'In vivo Toxicity Assessment of Silver Nanoparticles in Homeostatic versus Regenerating Planarians', Nanotoxicology, vol. 13, no. 4, pp. 476-491. https://doi.org/10.1080/17435390.2018.1553252

In vivo Toxicity Assessment of Silver Nanoparticles in Homeostatic versus Regenerating Planarians. / Leynen, Nathalie; Van Belleghem, Frank G.A.J.; Wouters, Annelies; Bove, Hannelore; Ploem, Jan-Pieter; Thijssen, Elsy; Langie, Sabine A.S.; Carleer, Robert; Ameloot, Marcel; Artois, Tom; Smeets, Karen.

In: Nanotoxicology, Vol. 13, No. 4, 21.04.2019, p. 476-491.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - In vivo Toxicity Assessment of Silver Nanoparticles in Homeostatic versus Regenerating Planarians

AU - Leynen, Nathalie

AU - Van Belleghem, Frank G.A.J.

AU - Wouters, Annelies

AU - Bove, Hannelore

AU - Ploem, Jan-Pieter

AU - Thijssen, Elsy

AU - Langie, Sabine A.S.

AU - Carleer, Robert

AU - Ameloot, Marcel

AU - Artois, Tom

AU - Smeets, Karen

PY - 2019/4/21

Y1 - 2019/4/21

N2 - Silver nanoparticles (AgNPs) belong to the most commercialized nanomaterials, used in both consumer products and medical applications. Despite its omnipresence, in-depth knowledge on the potential toxicity of nanosilver is still lacking, especially for developing organisms. Research on vertebrates is limited due to ethical concerns, and planarians are an ideal invertebrate model to study the effects of AgNPs on stem cells and developing tissues in vivo, as regeneration mimics development by triggering massive stem cell proliferation. Our results revealed a strong interference of AgNPs with tissue- and neuroregeneration which was related to an altered stem cell cycle. The presence of a PVP-coating significantly influenced toxicity outcomes, leading to elevated DNA-damage and decreased stem cell proliferation. Non-coated AgNPs had an inhibiting effect on stem cell and early progeny numbers. Overall, regenerating tissues were more sensitive to AgNP toxicity, and careful handling and appropriate decision making is needed in AgNP applications for healing and developing tissues. We emphasize on the importance of AgNP characterization, as we showed that changes in physicochemical properties influence toxicity.

AB - Silver nanoparticles (AgNPs) belong to the most commercialized nanomaterials, used in both consumer products and medical applications. Despite its omnipresence, in-depth knowledge on the potential toxicity of nanosilver is still lacking, especially for developing organisms. Research on vertebrates is limited due to ethical concerns, and planarians are an ideal invertebrate model to study the effects of AgNPs on stem cells and developing tissues in vivo, as regeneration mimics development by triggering massive stem cell proliferation. Our results revealed a strong interference of AgNPs with tissue- and neuroregeneration which was related to an altered stem cell cycle. The presence of a PVP-coating significantly influenced toxicity outcomes, leading to elevated DNA-damage and decreased stem cell proliferation. Non-coated AgNPs had an inhibiting effect on stem cell and early progeny numbers. Overall, regenerating tissues were more sensitive to AgNP toxicity, and careful handling and appropriate decision making is needed in AgNP applications for healing and developing tissues. We emphasize on the importance of AgNP characterization, as we showed that changes in physicochemical properties influence toxicity.

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KW - stem cell

KW - coating

KW - VITRO

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KW - STEM-CELL BIOLOGY

KW - DNA-DAMAGE

KW - ZINC-OXIDE

KW - MODEL

KW - PVP

KW - GENOTOXICITY

KW - DIFFERENTIATION

KW - CYTOTOXICITY

U2 - 10.1080/17435390.2018.1553252

DO - 10.1080/17435390.2018.1553252

M3 - Article

VL - 13

SP - 476

EP - 491

JO - Nanotoxicology

JF - Nanotoxicology

SN - 1743-5390

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