Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms

Michelle Plusquin, Katrien De Mulder, Frank Van Belleghem, Olivier DeGheselle, Nicky Pirotte, Maxime Willems, Ann Cuypers, Willi Salvenmoser, Peter Ladurner, Tom Artois, Karen Smeets*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Stem cells or undifferentiated cells can cope more easily with external stresses. To evaluate the impact of toxic compounds on stem cell dynamics in vivo, in relation to other biological responses, we use the carcinogenic element cadmium and the regenerating model organism Macrostomum lignano. Through both BrdU and anti-histone H3 immunostainings, cadmium-induced effects were investigated at different stages of the stem cell cycle. A 24-h exposure to 100 and 250 M CdCl2 significantly decreased the number of stem cells (neoblasts) in mitosis, whereas the number of cells in the S phase remained unchanged. After this short-term exposure, the ultrastructure of the neoblasts was minimally affected in contrast to the epidermal tissues. These results were supported by gene expression data: transcripts of cdc2 and pig3 were significantly upregulated during all treatments. Both genes are involved in the cell cycle progression and are transcribed in the gonadal region, where stem cells are highly represented. Based on a substantial increase in gene expression of heat shock proteins (HSP) and their high activity in the gonadal region, we hypothesize that these proteins are key players in the protection of stem cells against external stresses. Apart from the strong HSP induction, other protective processes including cell division, apoptosis and anti-oxidative defence, were also activated. We, therefore, conclude that the protection of stem cells against external stressors may be based on the interplay between stem cell maintenance, i.e. repair and recovery through division, on one hand and apoptosis on the other hand. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1217-1228, 2016.

Original languageEnglish
Pages (from-to)1217-1228
Number of pages12
JournalEnvironmental Toxicology
Volume31
Issue number10
Early online date6 Apr 2015
DOIs
Publication statusPublished - Oct 2016

Keywords

  • toxicology
  • stem cell
  • Macrostomum lignano
  • cadmium
  • stress
  • PLANARIAN SCHMIDTEA-MEDITERRANEA
  • POLYCELIS-FELINA DALY.
  • DNA-DAMAGE
  • MACROSTOMUM-LIGNANO
  • NEOBLAST DIVISION
  • REPAIR INHIBITION
  • OXIDATIVE STRESS
  • SELF-RENEWAL
  • PLATYHELMINTHES
  • EXPRESSION

Cite this

Plusquin, Michelle ; De Mulder, Katrien ; Van Belleghem, Frank ; DeGheselle, Olivier ; Pirotte, Nicky ; Willems, Maxime ; Cuypers, Ann ; Salvenmoser, Willi ; Ladurner, Peter ; Artois, Tom ; Smeets, Karen. / Toxic effects of cadmium on flatworm stem cell dynamics : A transcriptomic and ultrastructural elucidation of underlying mechanisms. In: Environmental Toxicology. 2016 ; Vol. 31, No. 10. pp. 1217-1228.
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title = "Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms",
abstract = "Stem cells or undifferentiated cells can cope more easily with external stresses. To evaluate the impact of toxic compounds on stem cell dynamics in vivo, in relation to other biological responses, we use the carcinogenic element cadmium and the regenerating model organism Macrostomum lignano. Through both BrdU and anti-histone H3 immunostainings, cadmium-induced effects were investigated at different stages of the stem cell cycle. A 24-h exposure to 100 and 250 M CdCl2 significantly decreased the number of stem cells (neoblasts) in mitosis, whereas the number of cells in the S phase remained unchanged. After this short-term exposure, the ultrastructure of the neoblasts was minimally affected in contrast to the epidermal tissues. These results were supported by gene expression data: transcripts of cdc2 and pig3 were significantly upregulated during all treatments. Both genes are involved in the cell cycle progression and are transcribed in the gonadal region, where stem cells are highly represented. Based on a substantial increase in gene expression of heat shock proteins (HSP) and their high activity in the gonadal region, we hypothesize that these proteins are key players in the protection of stem cells against external stresses. Apart from the strong HSP induction, other protective processes including cell division, apoptosis and anti-oxidative defence, were also activated. We, therefore, conclude that the protection of stem cells against external stressors may be based on the interplay between stem cell maintenance, i.e. repair and recovery through division, on one hand and apoptosis on the other hand. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1217-1228, 2016.",
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author = "Michelle Plusquin and {De Mulder}, Katrien and {Van Belleghem}, Frank and Olivier DeGheselle and Nicky Pirotte and Maxime Willems and Ann Cuypers and Willi Salvenmoser and Peter Ladurner and Tom Artois and Karen Smeets",
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Plusquin, M, De Mulder, K, Van Belleghem, F, DeGheselle, O, Pirotte, N, Willems, M, Cuypers, A, Salvenmoser, W, Ladurner, P, Artois, T & Smeets, K 2016, 'Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms', Environmental Toxicology, vol. 31, no. 10, pp. 1217-1228. https://doi.org/10.1002/tox.22129

Toxic effects of cadmium on flatworm stem cell dynamics : A transcriptomic and ultrastructural elucidation of underlying mechanisms. / Plusquin, Michelle; De Mulder, Katrien; Van Belleghem, Frank; DeGheselle, Olivier; Pirotte, Nicky; Willems, Maxime; Cuypers, Ann; Salvenmoser, Willi; Ladurner, Peter; Artois, Tom; Smeets, Karen.

In: Environmental Toxicology, Vol. 31, No. 10, 10.2016, p. 1217-1228.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Toxic effects of cadmium on flatworm stem cell dynamics

T2 - A transcriptomic and ultrastructural elucidation of underlying mechanisms

AU - Plusquin, Michelle

AU - De Mulder, Katrien

AU - Van Belleghem, Frank

AU - DeGheselle, Olivier

AU - Pirotte, Nicky

AU - Willems, Maxime

AU - Cuypers, Ann

AU - Salvenmoser, Willi

AU - Ladurner, Peter

AU - Artois, Tom

AU - Smeets, Karen

PY - 2016/10

Y1 - 2016/10

N2 - Stem cells or undifferentiated cells can cope more easily with external stresses. To evaluate the impact of toxic compounds on stem cell dynamics in vivo, in relation to other biological responses, we use the carcinogenic element cadmium and the regenerating model organism Macrostomum lignano. Through both BrdU and anti-histone H3 immunostainings, cadmium-induced effects were investigated at different stages of the stem cell cycle. A 24-h exposure to 100 and 250 M CdCl2 significantly decreased the number of stem cells (neoblasts) in mitosis, whereas the number of cells in the S phase remained unchanged. After this short-term exposure, the ultrastructure of the neoblasts was minimally affected in contrast to the epidermal tissues. These results were supported by gene expression data: transcripts of cdc2 and pig3 were significantly upregulated during all treatments. Both genes are involved in the cell cycle progression and are transcribed in the gonadal region, where stem cells are highly represented. Based on a substantial increase in gene expression of heat shock proteins (HSP) and their high activity in the gonadal region, we hypothesize that these proteins are key players in the protection of stem cells against external stresses. Apart from the strong HSP induction, other protective processes including cell division, apoptosis and anti-oxidative defence, were also activated. We, therefore, conclude that the protection of stem cells against external stressors may be based on the interplay between stem cell maintenance, i.e. repair and recovery through division, on one hand and apoptosis on the other hand. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1217-1228, 2016.

AB - Stem cells or undifferentiated cells can cope more easily with external stresses. To evaluate the impact of toxic compounds on stem cell dynamics in vivo, in relation to other biological responses, we use the carcinogenic element cadmium and the regenerating model organism Macrostomum lignano. Through both BrdU and anti-histone H3 immunostainings, cadmium-induced effects were investigated at different stages of the stem cell cycle. A 24-h exposure to 100 and 250 M CdCl2 significantly decreased the number of stem cells (neoblasts) in mitosis, whereas the number of cells in the S phase remained unchanged. After this short-term exposure, the ultrastructure of the neoblasts was minimally affected in contrast to the epidermal tissues. These results were supported by gene expression data: transcripts of cdc2 and pig3 were significantly upregulated during all treatments. Both genes are involved in the cell cycle progression and are transcribed in the gonadal region, where stem cells are highly represented. Based on a substantial increase in gene expression of heat shock proteins (HSP) and their high activity in the gonadal region, we hypothesize that these proteins are key players in the protection of stem cells against external stresses. Apart from the strong HSP induction, other protective processes including cell division, apoptosis and anti-oxidative defence, were also activated. We, therefore, conclude that the protection of stem cells against external stressors may be based on the interplay between stem cell maintenance, i.e. repair and recovery through division, on one hand and apoptosis on the other hand. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1217-1228, 2016.

KW - toxicology

KW - stem cell

KW - Macrostomum lignano

KW - cadmium

KW - stress

KW - PLANARIAN SCHMIDTEA-MEDITERRANEA

KW - POLYCELIS-FELINA DALY.

KW - DNA-DAMAGE

KW - MACROSTOMUM-LIGNANO

KW - NEOBLAST DIVISION

KW - REPAIR INHIBITION

KW - OXIDATIVE STRESS

KW - SELF-RENEWAL

KW - PLATYHELMINTHES

KW - EXPRESSION

U2 - 10.1002/tox.22129

DO - 10.1002/tox.22129

M3 - Article

VL - 31

SP - 1217

EP - 1228

JO - Environmental Toxicology

JF - Environmental Toxicology

SN - 1520-4081

IS - 10

ER -